Offline Sorter - Offline Spike Sorter and Data Analysis Software

Offline Sorter Data Sheet
Minimum System Requirements

Features

• Automatic cluster selection in feature space using valley seeking or T-Distribution E-M
• Manual cluster selection in 3D feature space using principal component projections, voltage slices in time, or more than 20 other wavform features
• Semi-automatic cluster selection in feature space using K-means or Standard E-M (Expectation Maximization)
• Waveform selection in time-voltage space
• Template matching algorithm
• Tetrode and Stereotrode waveform extraction and sorting using any method
• Unit cross-correlograms and ISI plots
• Waveform density plots in 2D or 3D in any feature space
• Waveform alignment
• Interval invalidation and artifact removal
• Raster displays of spikes and continuous data
• Printable sorting summary view with export to PowerPoint®
• Sort summary statistics
• Waveform features and statistics that can be easily exported to MATLAB®, Excel® or text file
• Export of sorted data to Neuroexplorer™ or optimized binary format (for fast loading)

Description

Offline Sorter (OFS) speeds up the process of selecting and classifying action potential waveforms (spikes) collected from single electrodes or stereotrodes/tetrodes. Spikes can be displayed as points in either 2D or 3D feature space, where a variety of manual, semi-automated, or fully automated clustering techniques can be applied in order to classify (sort) the spikes. OFS can also perform spike extraction on continuously-recorded neural data using several different thresholding methods. OFS allows verification of sorting through a variety of displays, and can calculate statistics and sort quality metrics. The sorted spikes can be exported in several formats for subsequent analysis.

Multiple methods for spike detection in continuously digitized data files can be used, including:

• Voltage threshold
• Signal energy (~ voltage2 )
• Nonlinear energy

Stereotrode and tetrode spike detection is supported. A low-cut filter may be applied to the continuous data prior to spike extrtraction.

The typical analysis pathways and files related to OFS are shown in the diagram below.

Offline Sorter can be used to sort or re-sort data files containing either thresholded waveform segments or continuously digitized data. File types supported include:

• Plexon
• NeuroExplorer
• Cyberkinetics
• CED Spike-2
• DataWave
• Neuralynx, Inc.
• Multi Channel Systems
• Panasonic MED
• Neuroshare
• Generic binary continuous data

Requirements:
The minimum system requirements:

• PC with Pentium III or better CPU
• Windows XP or XP64, Windows 2000, or Windows Vista (32-bit)
• At least 1 GB RAM recommended
• Modern graphics subsystem with OpenGL support
• Plexon hardware license key
  

For ordering information contact Plexon Sales at (214) 369-4957 or info@plexoninc.com. You may also download a fully functional demo version from the Plexon site.