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Offline Sorter v3 -
Offline Spike Sorter and Data Analysis Software
Features
Powerful Waveform Viewing and Sorting
- Manual cluster selection in 3D feature space using principal
component projections, voltage slices in time, or more than
20 other waveform features such as peak, valley, full-width
at half maximum, etc.
- Box sorting (like in the Sort Client on-line MAP control
software)
- Waveform selection in time-voltage space
- Template matching algorithm
- Tetrode and Stereotrode waveform extraction and sorting using
any method
- Unit cross-correlograms and ISI plots
- Waveform density plots in 2D or 3D in any feature space
- Waveform alignment
- Interval invalidation and artifact removal
- Raster displays of spikes and continuous data
- Printable sorting summary view with export to PowerPoint®
- Sort summary statistics
- Waveform features and statistics that can be easily exported
to MATLAB®, Excel® or text file
- Export of sorted data to Neuroexplorer™ or optimized
binary format (for fast loading)
- Save and recall particular sorting instances for a selected
channel (useful for comparisons)
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Time segmentation, adaptive sorting
methods, and time-dependent analysis
- Divide each file into time segments and view and sort
waveforms from each time segment separately
- Clusters vs. Time view to show how clusters evolve
as a function of time
- Sort Quality vs. Time Segment graph to display how
the sort quality changes through the file
- Adaptive template sorting, with the templates changing
over time to follow the waveform as it evolves
- Template vs. Time view to display how the template
adapted through the file
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Semi-Automatic and Automatic Sorting
- Enhanced automatic sorting methods with systematic scanning
through parameter space and graphs displaying the sort quality
metric as a function of the parameters that were varied;
select the best sorting according to a selected sort quality
metric
- Automatic sort scan results for each channel can be saved
to and loaded from .SCAN files. This, together with the new
batch mode scan commands, will allow the running of certain
sorting scans overnight, followed by manual reviewing and
selection of the most appropriate sort for each channel
- Automatic cluster selection in feature space using valley
seeking or T-Distribution E-M
- Semi-automatic cluster selection in feature space using
K-means or Standard E-M (Expectation Maximization)
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Enhanced Batch Mode Processing for Running Sorting
Algorithms Overnight
- New batch mode commands for scanning through automatic sorting
parameters
- New menu item to edit batch files with a user-selected editor
- Memory of previous batch file locations
- New Quick Batch Reference showing all batch commands
- New menu item showing the log file resulting from the latest
batch run
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Enhanced Continuous Data Handling and Spike Detection
- Up-sampling of continuous signals, with linear or spline
interpolation
- FFT view of continuous data
- Single and Dual Thresholds for spike detection
- Waveform detection and extraction now appropriately handles
differences in voltage scales between spike waveform segments
and continuous spike data
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Improved User Interface, Displays, and Performance
- Enhanced bottom Timeline View showing both continuous and
waveform data simultaneously with a resizable splitter
- Mouse-over time and voltage readings in the waveform and
continuous data views
- More information related to gains and voltage ranges in the
Channel Parameters and File Summary views
- New Select main menu item to select the next/previous channel,
unit, waveform, or time segment
- Performance enhancements for faster loading data files
- Now available as a true 64-bit executable – for faster
processing of large data files
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Description
Offline Sorter (OFS) version 3 is a powerful, easy-to-use
tool for viewing and classifying action potential waveforms (spikes)
collected from single electrodes, stereotrodes, and tetrodes. Spikes
can be displayed as points in either 2D or 3D feature space, where
a variety of manual, semi-automated, or fully automated clustering
techniques can be applied in order to classify (sort) the spikes. OFS
can also perform spike extraction on continuously-recorded neural data
using a variety of different thresholding methods. OFS allows verification
of sorting through a variety of displays, and can calculate cluster
separation statistics and sort quality metrics. The sorted spikes can
be exported in several formats for subsequent analysis.
The parameter
space for several fully automatic spike sorting algorithms can be
systematically scanned through, with graphs displaying the sort quality
metric as a function of the parameters varied. The results of the automated
scanning through sorting parameter space can be saved in a .SCAN file.
This, combined with new batch mode commands, allow the running of various
sorting algorithms overnight, followed by rapid manual viewing of
different sort outcomes and selection of the appropriate sort for
each channel.
Offline Sorter version 3 also contains new time
segmented views of the waveforms, with cluster vs. time and sort quality
vs. time views that show how the waveforms change and evolve over time.
Multiple methods for spike detection in continuously
digitized data files can be used, including:
- Voltage threshold
- Signal energy (~ voltage2)
- Nonlinear energy
Stereotrode and tetrode
spike detection is supported. A low-cut filter may be applied to
the continuous data prior to spike extratraction.
The typical analysis
pathways and files related to OFS are shown in the diagram below.

Offline Sorter can be used to sort or re-sort data
files containing either thresholded waveform segments or continuously
digitized data. File types supported include:
- Plexon
- NeuroExplorer
- Cyberkinetics
- CED Spike-2
- DataWave
- Neuralynx, Inc.
- Multi Channel Systems
- Panasonic MED
- Neuroshare
- Generic binary continuous data
Requirements:
The minimum system requirements:
- PC with Pentium III or better CPU
- Windows XP or XP64, Windows 2000, or Windows
Vista (32-bit or 64-bit)
- At least 1 GB RAM recommended
- Modern graphics subsystem with OpenGL support
- Plexon hardware license key
For ordering information contact Plexon Sales at
(214) 369-4957 or info@plexoninc.com. You may also download a fully
functional demo version from the Plexon site.
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